Serratia Marcescens Lab Report Essay Example

Serratia Marcescens Lab Report Paper In bacteria, temperature, pH, and other chemical agents a II affect the expression of genes. In this lab, the effect of temperature change on the gene which codes for a red pigment called prognosis of bacterium Seer TIA mercenaries is being tested. Seriate mercenaries is usually found in OSI I and plants, and the accumulation of prognosis in the bacterial cells makes them appear red. Prognosis is produced only at certain temperatures, so b y regulating the temperature in which Seriate mercenaries is cultured, the optimum temperature for the most prognosis to be produced can be tested. Purpose The purpose of this lab is to observe the effect of temperature c anger on the production of the pigment prognosis by the back terbium Seriate mercenaries, and also to determine whether previous culture conditions affect gene expression. Hypothesis If the bacteria is cultured in 27 , then it will produce more prognosis than the bacteria cultured in 37 Regardless of the first culture conditions, the bacteria recaptured in 27 co will produce more prognosis than the bacteria recaptured in 37 Materials see attached lab, materials, page 25 Independent Variable: Temperature (in Celsius) Dependent Variable: Amount of prognosis produced Methods/Procedures see attached lab, procedures, page 2527 Data see attached lab, data, page 25, 26 Analysis 1. You should label the bottom off Petri dish instead of the top because this way, the lids will not accidentally be sit ached. We will write a custom essay sample on Serratia Marcescens Lab Report specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Serratia Marcescens Lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Serratia Marcescens Lab Report specifically for you FOR ONLY $16.38 $13.9/page Hire Writer It is also easier to read the label if it is on the bottom, because the Petri dishes will be put into the incubator upside down to minimize condensation. 2. You must not touch a non sterile surface with the applicant or tip before obtaining the innocuous from the stock culture because the applicator tip might become contaminated, which would compromise the experiment. 3. You should lift the lid of the Petri dish only 23 CM rather t Han remove it completely in order to keep as little of any thing other than the bacteria from contaminating or getting into the Petri dish. . If I cultured two samples of bacteria and grew them at 32 co , I predict that the samples will produce less prognosis (be less red) than the bacteria cultured in 27 co , but produce more prognosis (be more red) than the bacteria cultured in 37 co 5. If the new cultured were grown at 37 for 8 hours, then at 27 for 24 hours, I predict hat the bacteria will produce prognosis (be red) because according to the data, the c onditions of the recapture have more of an effect on the production of prognosis than the conditions of the initial culture. . The temperature at which the bacteria were originally re cultured has more effect on the production of prognosis t Han the temperature at which the bacteria were originally cultured. Both samples recaptured in 27 were red, meaning that they produced prognosis, regardless of their initial culture c notations, while both samples recaptured at 37 ere white, signifying the absence of prognosis production, also regardless of their original culture conditions. 7. Prognosis is not only a pigment; it is also an antibiotic. Its function may be to kill other microorganisms which might be harmful to the Seriate mercenaries that live in the same temperature range. 8. An advantage of the temperature sensitivity of prognosis proud action might be that the bacteria would only produce it when needed d. The ability to control the production of prognosis according to temperature helps the bacteria to synthesize the pigment helps it o not produce excess prognosis when it is not needed. Conclusion My hypothesis, which was that the bacteria cultured in 27 co will produce more prognosis than the bacteria cultured in 37 , and also that the bacteria recaptured in 27 (regardless of the original culture conditions) will produce more p Rodings than the bacteria recaptured in 37 (also regardless of the original culture condition s), was supported. The S. Mercenaries cultured in 27 turned red, indicating the production of pro disposing, while the S. Mercenaries cultured in remained white, indicating that no prognosis was synthesized.